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Image Search Results
Journal: PLoS ONE
Article Title: IKAP Deficiency in an FD Mouse Model and in Oligodendrocyte Precursor Cells Results in Downregulation of Genes Involved in Oligodendrocyte Differentiation and Myelin Formation
doi: 10.1371/journal.pone.0094612
Figure Lengend Snippet: ( A ) Western blot analyses of total protein lysates from brain cortex of control (N1, N2, N3) and age-matched FD (FD1, FD2, FD3) littermates. Upper panel shows detection of IKAP with the polyclonal anti-IKAP antibody (AnaSpec), and lower panel shows anti-β-actin for loading control. Note that IKAP protein expression is highly reduced in FD1, FD2, and FD3, relative to controls. Quantitative analysis of the Western blot was performed using Image J software. IKAP expression levels over β-actin levels are presented. ( B ) Western blot analyses of total protein lysates from control scrambled (ShSCR) and IKAP knockdown (ShIKAP) Oli-neu cells. Upper panel shows detection of IKAP with the polyclonal anti-IKAP antibody (AnaSpec), and lower panel shows anti-β-actin for loading control. Note that IKAP is significantly expressed in ShSCR Oli-neu cells. Quantitative analysis of the Western blot was performed using Image J software. IKAP expression levels over β-actin levels and are presented.
Article Snippet: Primary antibodies specific for
Techniques: Western Blot, Control, Expressing, Software, Knockdown